ANALYSIS OF THE CONTENT OF HEAVY METAL SALTS IN MILK AND THEIR INTERACTION WITH ENZYMES

The article substantiates the relevance of the application aimed at studying the content of heavy metals in milk and their interaction with enzymes. It should be noted that research is currently underway to study the interaction of enzymes with heavy metals in environmental objects and in a living organism. It is known that certain heavy metals both inhibit the activity of individual enzymes and stimulate their activity. For example, studies have been conducted proving that cadmium stimulates peroxidase activity, but inhibits catalase activity regardless of the concentration of this element in the soil. The main purpose of the study was to study the interaction of heavy metal salts with redox groups of the milk enzyme. When studying the interaction of salts of lead, cadmium, arsenic and mercury with catalase and peroxidase, it was found that the salts of the studied heavy metals have only an inhibitory effect on the enzyme catalase and peroxidase. At the same time, salts of elements such as mercury and cadmium have the greatest inhibitory effect, followed by lead and arsenic. The restoration of milk peroxidase activity was established after the introduction of oatmeal and additional filtration by qualitative reaction. With a lead content of 0.06 mg/l in the milk sample, catalase activity was 4.98 E, and after filtration, its activity increased to 8.3 E. With a cadmium content of 0.20 mg/l in milk, catalase activity before filtration was 3.32 E, after filtration it increased to 5.81 E. With a mercury content of 0.011 mg/l in milk, catalase activity before filtration was 2.49 E, after filtration it increased to 4.15 E. With an arsenic content of 0.11 mg/l in milk, catalase activity was 6.64 E, after filtration it increased to 8.3 E. Currently, research is underway to study the interaction of enzymes with heavy metals in environmental objects, in a living organism, but the issue of research on the interaction of heavy metals with milk enzymes has not been studied. 

1. Novoselova E.I. Vliyanie zagryazneniya kadmiem na fermentativnuyu aktivnost' chernozema obyknovennogo / E.I. Novoselova, S.A. Bashkatov // Vestnik Bashkirskogo universiteta. – 2014. – T. 19, № 4. – S. 1204-1207. (In Russian).

2. Dobriyan E.I. Antioksidantnaya sistema moloka / E.I. Dobriyan // Vestnik VGUIT. – 2020. – T. 82, № 2. – S. 101-106. (In Russian).

3. Zhidkin V.I. Radioaktivnye zagryazneniya pishchevykh produktov, ikh posledstviya dlya zdorov'ya cheloveka i radiozashchita pitaniem / V.I. Zhidkin, T.I. Sul'dina // Materialy Mezhdunar. nauch.-prakt. konf. v 2 chastyakh – Saransk, 2014. – S. 118-122. (In Russian).

4. Zhidkin V.I. Puti zagryazneniya prodovol'stviya / V.I. Zhidkin, A.M. Semushev // Tret'i chteniya pamyati professora O.A. Zauralova: materialy Mezhdunar. nauch.-prakt. konf., Saransk, 13 maya 2011 g. – Saransk, 2011. – S. 20-23. (In Russian).

5. Perspektivnye napravleniya primeneniya tseolita dlya ochistki moloka ot toksikoehlementov / A.K. Kakimov i dr. // Tekhnika i tekhnologiya pishchevykh proizvodstv. – 2018. – Tom 48, № 1. – S.143149. (In Russian).

6. Uzhakhova L.YA. Issledovanie moloka na soderzhanie tyazhelykh metallov / L.YA. Uzhakhova // Colloquium-journal. – 2018. – № 4-2(15). – S. 60-61. (In Russian).

7. Assessment of heavy metal contamination in raw milk for human consumption / M. Younus et al // South African journal of Animal Science. – 2016. – Vol. 46, № 2. – P. 166-169. (In English).

8. Knowledge and acceptance of functional foods: A preliminary study on influence of a synbiotic fermented milk on athlete health/ M.M. Coman et al // International Journal of Probiotics and Prebiotics. – 2019. – Vol. 12, № 1. – P. 33-41. (In English).

9. Marynchenko L. Research of mineral adsorbents application for water-alcohol solutions purification in technology of alcoholic beverages / L. Marynchenko, V. Marynchenko, M. Hyvel // EUREKA: Physics and Engineering. – 2018. – № 4. – P. 3-10. (In English).

10. Agbugui A. Levels of Some Heavy Metals in Fresh Cow Milk obtained from Herder settlement around Okada, Benin City, Edo State Nigeria. / A. Peter Agbugui, C. Luke Orjekwe, S. Abiodun Solola // IOSR Journal of Applied Chemistry (IOSR-JAC). – 2020. – Volume 15, Issue 6 Ser. II – P. 23-29. (In English).

11. Investigation of heavy metal contents in Cow milk samples from area of Dhaka, Bangladesh / M.I. Muhib et al // International journal of Food Contamination. – 2016, 3:16. (In English).