Preview

Bulletin of Shakarim University. Technical Sciences

Advanced search

TECHNOLOGY FOR MAKING GREEK YOGURT AND DETERMINING THE AMOUNT OF PROTEIN IN ITS WHEY

https://doi.org/10.53360/2788-7995-2025-1(17)-38

Abstract

This scientific article discusses the technology for the production of Greek yogurt using skim milk and adding medium-chain triglyceride (MCT) fat. The emphasis is on the analysis of the protein content of the whey obtained after removing excess liquid from the yogurt. The purpose of the study is to determine the amount of whey protein and assess the possibilities of its further use for enriching the finished product. The protein determination process was carried out by the Bradford method using a spectrophotometer, which made it possible to quantitatively analyze the amount of protein in the remaining serum. Bradford's method is based on the interaction of proteins with the kumassi dye Diamond Blue G-250, which binds to proteins and changes the color of the solution, which makes it possible to measure protein concentration by optical density. The results of the study show that whey proteins have a high potential for reuse. Enriching Greek yogurt with these proteins can increase its nutritional value by increasing the protein content. Thus, an innovative approach is proposed to use the by-product of the production of Greek yogurt, which helps to increase the efficiency of the production process and reduce food waste. The results can be useful for dairy industry enterprises engaged in the production of fermented milk products, as well as for scientific research in food technology and nutritiology. The use of this method of analysis and processing of whey proteins contributes to the creation of products of high biological value and reducing production costs. Thus, the proposed technology is a promising direction for further research and implementation in industrial production. 

About the Authors

S. Toleubekova
Shakarim University of Semey
Kazakhstan

Sandugash Toleubekova – Candidate of Technical Sciences, Associate Professor; Department of Biotechnology

071412, Semey city, Glinka street 20A



A. Alipov
Shakarim University of Semey
Kazakhstan

Adil Alipov – master of the 2nd year of the specialty «Standardization and certification»

071412, Semey city, Glinka street 20A



Ye. Zharykbasov
Shakarim University of Semey
Kazakhstan

Yerlan Zharykbasov – PhD; Department of Biotechnology

071412, Semey city, Glinka street 20A



M. Jumazhanova
Shakarim University of Semey
Kazakhstan

Madina Jumazhanova – PhD; Department of Biotechnology

071412, Semey city, Glinka street 20A



A. Kabdenova
Shakarim University of Semey
Kazakhstan

 Ainur Kabdenova Master of Biotechnology; Department of Biotechnology

071412, Semey city, Glinka street 20A



References

1. Kaishev V.G. Sostoyanie i perspektivy razvitiya rynka funktsional'nykh produktov pitaniya / V.G. Kaishev, S.N. Sergin // Pererabotka moloka. – 2018. – № 2. – S. 64-67. (In Russian).

2. Onecia B. Chronic Pancreatitis / B. Onecia, L.L. Sarah. – 2022. https://www.ncbi.nlm.nih.gov/books/NBK482325/. (In English).

3. Petrov V.N. Khronicheskii kholestsestit / V.N. Petrov, V.A. Lapotnikov // Sankt-Peterburgskaya meditsinskaya akademiya poslediplomnogo obrazovaniya, Sankt-Peterburgskii gosudarstvennyi meditsinskii universitet im. akad. Pavlova. – 2011. (In Russian).

4. Nutrition Guidelines for Chronic Pancreatitis Patient Education // Digestive Health Center at Stanford Hospital and Clinics // NS 12/2012. (In English).

5. Chapman-Lopez T.J. The Effects of Medium-Chain Triglyceride Oil Supplementation on Endurance Performance and Substrate Utilization in Healthy Populations: A Systematic Review / T.J. Chapman-Lopez, Yu. Koh // PMCID: PMC9579472 PMID: 36096496. – 2022. – № 31(3). – R. 217-229. (In English).

6. Medium-Chain Triglyceride Oil and Blood Lipids: A Systematic Review and Meta-Analysis of Randomized Trials / K.M. McKenzie et al // The Journal of Nutrition. – 2021. – Vol. 151, Issue 10. – P. 2949-2956. (In English).

7. Separation Technologies for Whey Protein Fractionation / G.Q. Chen et al. 2023. – Vol. 15. – R. 438-465. (In English).

8. Ganju S. A review on approaches for efficient recovery of whey proteins from dairy industry effluents / S. Ganju, PR. Gogate // J Food Eng. – 215. – R. 84-96. (In English).

9. Membrane processes for whey proteins separation and purification a review / R. Aguero et al // Curr Org Chem. – № 21(17). – R. 1740-1752. (In English).

10. Zydney AL. Protein separations using membrane filtration: new opportunities for whey fractionation / AL. Zydney // Int Dairy J. – № 8(3). – R. 243-250. https://doi.org/10.1016/s09586946(98)00045-4. (In English).

11.


Review

For citations:


Toleubekova S., Alipov A., Zharykbasov Ye., Jumazhanova M., Kabdenova A. TECHNOLOGY FOR MAKING GREEK YOGURT AND DETERMINING THE AMOUNT OF PROTEIN IN ITS WHEY. Bulletin of Shakarim University. Technical Sciences. 2025;(1(17)):298-304. (In Kazakh) https://doi.org/10.53360/2788-7995-2025-1(17)-38

Views: 70


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2788-7995 (Print)
ISSN 3006-0524 (Online)
X