USE OF ENZYMES FOR DETERMINATION OF FOOD SAFETY

The article presents the results of the study of determination of specific activity of acetylcholinesterase enzyme. For the immobilisation of enzymes in the development of biosensor test systems for the determination of phosphorus-organic pesticides in environmental objects the researchers used mainly enzymes from the class of hydrolases, namely, acetylcholinesterase or butyrylcholinesterase, as a biocatalyst. To justify the choice of enzyme in the development of the test system, studies were carried out to determine the specific activity of two hydrolytic enzymes: acetylcholinesterase and butyrylcholinesterase.

The paper applied theoretical and experimental studies. Experimental studies were carried out on the basis of generally accepted, modified and standard research methods of physicochemical, organoleptic, rheological, hygienic safety indicators of research objects, as well as specific activity of enzymes.

Since milk is a complex polydisperse system, an important factor is the choice of an enzyme with higher specific activity. According to literature sources, hydrolytic enzymes (acetylcholinesterase and butyrylcholinesterase) are highly sensitive to the inhibitory properties of carbophos, which is manifested by a decrease in the specific activity of the enzyme.

The results of experimental studies showed that of the two hydrolytic enzymes acetylcholinesterase shows the highest specific activity in comparison with butyrylcholinesterase both in aqueous medium and in milk.

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