OPTIMIZING THE EXTRACTION METHOD OF TANNINS FROM ALHAGI KIRGISORUM SCHRENK AND THEIR BIOACTIVITY

Plants are a raw material source for obtaining various biologically active complexes, and their isolation can be used to obtain new drugs with diverse biological activities. As sources of biologically active substances, plants of the genus Alhagi, the species Alhagi Kirgisorum Schrenk (zhantak – Kyrgyz camel thorn), are of great interest. Alhagi Kirgisorum Schrenk, which was collected during the flowering period in the Shelek district, Almaty region, in 2023, is an aboveground part of the plant species. Analysis of biologically active substances in plant raw materials, consideration of effective technological parameters for extracting biologically active complexes from plants using ultrasonic extraction, and study of the pharmacological activity of the obtained medicinal preparations are important issues. In order to isolate the complex of tannins from plant raw materials: 50% ethanol solvent and ultrasonic extraction and maceration methods, a ratio of raw materials: extractant – 1:8 was proposed. The composition of this plant is very rich in secondary metabolites, including tannin substances, and it is known that their share is 4,92%. The complex obtained by using two different methods: maceration and ultrasonic extraction was subjected to component analysis, and it was found that the extract obtained by ultrasonic extraction contained more tannin substances. The anticholinesterase biological activity of the complex showed 48,20±4,39% against AChE, 4,21±0,39% against BChE, antidiabetics by the α-Amylase inhibitory activity against was 2807,63±0,10, α-Glucosidase inhibitory activity was 183,51±0,80. Also, against oxidation process: ABTS+ assay 12,52±2,16 (6,25-50 µg/mL), DPPH assay 15,70±0,46 (3,125-25 µg/mL), CUPRAC 20,82±0,02 (6,25-50 µg/mL) and according to β- carotene/linoleic acid analysis showed 38,73±0,75 (3,125-25 µg/mL) activity.

1. Alhagi: a plant genus rich in bioactives for pharmaceuticals / G. Muhammad et al // Phytotherapy research. – 2015. – V.29, №1. – Р. 1-13. https://doi.org/10.1002/ptr.5222. (In English).

2. Rakhimov K.D. Farmakologicheskoe i khimiko-farmatsevticheskoe issledovanie lekarstvennykh sredstv rastitel'nogo proiskhozhdeniya iz Alkhagi kirgizskoi (Alhagi kirgisorum Schrenk) / K.D. Rakhimov, G.Sh. Burasheva, G.O. Ustenova. – Almaty, 2018. – 394 s.http://e-kitap.kz/book/4866. (In Russian).

3. Alhagi kirghisorum schrenk: technological aspects of its thick extract for the pharmaceutical application / Н. Kukhtenko et al // Agrobiodivers Improv Nutr Health Life Qual. – 2022. – № 6(1). – Р. 75-84. https://doi.org/10.15414/ainhlq.2022.0009 .(In English).

4. Analysis of the current state of the pharmaceutical market of the republic of Kazakhstan / K. Zhakipbekov et al // ScienceRise: Pharmaceutical Science. – 2023. – V. 42, № 2. – 57 P. https://doi.org/10.15587/2519-4852.2023.267787. (In English).

5. Alchera F., Ginepro M., Giacalone G. Microwave-assisted extraction of polyphenols from blackcurrant by-products and possible uses of the extracts in active packaging // Foods. – 2022. – V.11. No.18. – 2727 p. https://doi.org/10.3390/foods11182727. (In English).

6. Optimization of microwave-assisted extraction of phenolic compounds from chestnut processing waste using response surface methodology / I.T. Tomasi et al // Journal of Cleaner Production. – 2023. – V. 395. – 136452 p. https://doi.org/10.1016/j.jclepro.2023.136452. (In English).

7. Response Surface Methodology for the Optimization of Flavan-3-ols Extraction from Avocado ByProducts via Sonotrode Ultrasound-Assisted Extraction / M.D. Razola-Díaz et al // Antioxidants. – 2023. – V. 12, № 7. – 1409 P. https://doi.org/10.1016/j.ultsonch.2021.105773. (In English).

8. Antioxidant and anti-diabetic properties of olive (Olea europaea) leaf extracts: In vitro and in vivo evaluation / H.M. Mansour et al // Antioxidants. – 2023. – V.12, № 6. – 1275 P. https://doi.org/10.3390/antiox12061275. (In English).

9. Determination of the total phenolics content and antioxidant activity of extracts from parts of plants from the Greek Island of Crete / E. Kalpoutzakis et al // Plants. – 2023. – V.12, № 5. – 1092 p. https://doi.org/10.3390/plants12051092. (In English).

10. Chemical composition and biological activities of essential oils and extract of Eucalyptus citriodora Hook / B. Şahin et al // International Journal of Secondary Metabolite. – 2024. – V. 11, № 2. – Р. 394-407. https://doi.org/10.21448/ijsm.1384581. (In English).