SCREENING OF XYLANASE PRODUCING STRAINS AND ITS APPLICATION IN FLUE-CURED TOBACCO LEAF FERMENTATION

In this experimental study, a strain producing xylanase extracted from tobacco leaf was developed. Through the basic strain screening method and xylanase activity determination, strain M416 with the highest enzyme activity was preliminarily identified and M416 was A. niger, the enzyme has biological safety and can be used in the fermentation process of flue-cured tobacco. The fermentation conditions were optimized by shaking the flask culture, in which the best formulation of xylanase production from A. niger and the optimal culture temperature, pH, inoculum amount and culture time were obtained. Under the optimal fermentation and aroma production conditions, the strain are evenly sprayed to the surface of tobacco leaf to determine the changes of the composition and content of aroma substances in the fermentation process, and evaluate the influence of xylanase producing niger on the aroma composition in the process of tobacco leaf fermentation, which shows that the strain can effectively increase the content of aroma substances.

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