STUDY OF THE IMMOBILIZATION OF THE PROBIOTIC STRAIN LACTOBACILLUS PARACASEI-010-K ON A NATURAL SORBENT FOR USE IN POULTRY FARMING

This article presents the results of a study on the immobilization of cells of the probiotic strain Lactobacillus paracasei-010-K on natural adsorbents.
Methods of cell immobilization can increase their stability, reduce the lethal effect of microorganisms on cells during their passage through the gastrointestinal tract, improve visceral parameters and clinical outcomes compared with free cells. The local mineral vermiculite was used to immobilize lactic acid bacteria. The sample is characterized by a high content of macro and microelements compared to samples of vermiculites from other deposits in the country and it has high adsorption activity (20-50% by volume). To immobilize the cells of the strains, the mineral adsorbent vermiculite was added in amounts of 0.25, 0.5, 0.75 and 1.0 (%) to daily cultures of lactic acid bacteria with titers of 1*10⁷ CFU/ml in the MRS (de Man, Rogosa and Sharpe agar) nutrient medium. The adsorption-immobilization process was carried out at 37°C with stirring for 24 hours. Liquid preparations containing lactobacillus cells and adsorbent were stored at a temperature of 4-6°C for 30, 60 and 90 days to assess the stabilizing effect of the adsorbent and determine its optimal amount. In the course of studies on the immobilization of microorganisms on enterosorbents, it was found that vermiculite does not adversely affect the bioactivity and viability of lactic acid bacteria cells. Moreover, when vermiculite was introduced into the MRS medium at a concentration of 0.5%, the titer of lactic acid bacteria increased to 1*10⁹ CFU/ml. The resulting preparations containing cultures of lactic acid bacteria are further used to introduce them into the composition of feed additives for poultry farming.

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