STUDY METHODS OF INFLUENCE OF OIL ON DIFFERENT GROUPS OF MICROORGANISMS AND ENVIRONMENTAL EVALUATION OF GEOSYSTEM OBJECTS

The article is devoted to the study of the influence of oil on various groups of microorganisms involved in the nitrogen cycle and the environmental assessment of geosystems. During all periods of the study, the number of azofixing microorganisms and oligonitrophils in the contaminated soil was higher than uncontaminated. An increase in the intensity of nitrogen fixation in polluted soil may be due to an increase in the content of readily available organic matter – alcohols, organic acids, amino acids, and other compounds. It correlates with an increase in the number of groups of anaerobic nitrogen fixers of the genus Clostridium. It is possible that nitrogen-fixing microorganisms may use metabolic products of hydrocarbon-oxidizing microorganisms. An increase in the soil contaminated with oil of nitrogen-fixing microorganisms is noted by a number of researchers.

The most sensitive to oil pollution are nitrifying bacteria, which are an agronomically important group of microorganisms. Their numbers fell with an increase in the concentration of oil and did not recover to the background level after a certain time and when used for the remediation of contaminated soils, as a complex of fertilizers, and activated sludge and other stimulants.

Microbiological examination of oil-contaminated sites was carried out according to generally accepted methods in microbiological practice. Separation of the YOM was carried out on MPA and Voroshilova-Dianova, Koch methods and various fractions of oil products were used as petroleum products for carbon nutrition of microorganisms.

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