Preview

Bulletin of Shakarim University. Technical Sciences

Advanced search

MICROBIAL DIVERSITY OF TRADITIONAL FERMENTED DAIRY PRODUCTS: SPECIES CHARACTERIZATION AND BIOTECHNOLOGICAL PROSPECTS FOR LAMB FERMENTATION

https://doi.org/10.53360/2788-7995-2025-3(19)-49

Abstract

The use of starter cultures with active metabolic activity during fermentation represents an effective biotechnological method for processing meat products, ensuring their safety, enhancing microbiological stability, and extending shelf life. A particularly promising approach involves the application of strains isolated from traditional fermented dairy products, which contribute to preserving authentic flavor and improving the organoleptic characteristics of fermented lamb. The development of domestic starter cultures based on such strains is of practical importance for expanding the range of national meat products, reducing dependence on imported cultures, and aligning with the requirements of the modern food industry and the concept of functional nutrition.
The aim of this study is to conduct a comparative microbiological analysis of the traditional fermented dairy product kurt, produced using different technological recipes, and to assess the influence of fermentation duration on the quality characteristics of lamb when using starter cultures based on isolated bacteria.
In this work, lactic acid bacteria were isolated and preliminarily identified from three samples of the traditional Kazakh fermented dairy product kurt. Strains of Lactobacillus casei and Lactococcus lactis, which demonstrated high viability and favorable organoleptic properties upon activation, were used as starter cultures. A fermentation technology for lamb was developed using these microorganisms isolated from kurt.
Meat samples were incubated at 4 °C with the addition of 5% salt and 20% starter culture for 4, 8, and 12 hours. The results indicated a decrease in pH, an increase in moisture and fat content, improvement in texture, and the formation of a meat-dairy aroma. Water activity remained at a microbiologically safe level (Aw < 0.90), ensuring product stability.
Both theoretical and experimental research methods were employed in the study. Experimental work was conducted at the Laboratory of Physicochemical Analysis of Food Products named after Professor K. Z. Amirkhanov at Shakarim University, using conventional, modified, and standardized methodologies.
The results confirm the potential of using autochthonous lactic acid cultures derived from kurt in the production of traditional fermented lamb products, contributing to improved quality, safety, and sensory characteristics. Future research should focus on optimizing the fermentation process and enhancing the functional properties of the final product.

About the Authors

M. Sharapatova
Toraighyrov University of Pavlodar
Kazakhstan

Madina Sharapatova – doctoral student of the Department of Biotechnology , Toraighyrov University of Pavlodar 

140000, Republic of Kazakhstan, Pavlodar, Lomova st.64



Z. Kapshakbayeva
Toraighyrov University of Pavlodar
Kazakhstan

Zarina Kapshakbayeva – PhD, Associate Professor of the Department of Biotechnology

140000, Republic of Kazakhstan, Pavlodar, Lomova st.64



K. Issayeva
Toraighyrov University of Pavlodar
Kazakhstan

Kuralay Issayeva – Candidate of Technical Sciences, Associate Professor of the Department of Biotechnology

140000, Republic of Kazakhstan, Pavlodar, Lomova st.64 



References

1. International Journal of Food Science and Technology / Integrating Tradition and Technology in National Food Production: A Global Perspective. – 2021. – Vol. 56, Issue 9, R. 1247-1263. (In English).

2. Kochkorova F.A. Nutrional value of the national dairy product kurut and its place in the nutrition of adolescents of the Kyrgyz Republic / F.A. Kochkorova, G.S. Kitarova // Voprosy pitaniia [Problems of Nutrition]. – 2021. – № 90(5). – R. 87-95. https://doi.org/10.33029/0042-8833-2021-90-5-87-95. (In English).

3. Ermolaeva A.N. Vydelenie i izuchenie promyshlenno- tsennykh shtammov mikroorganizmov iz kislomolochnykh produktov // Biotekhnologiya. Teoriya i praktika. – 2012. – № 3. (In Russian).

4. Kurgambaeva G.S. Vydelenie i identifikatsiya drozhzhei roda Saccharomyces iz kumysa razlichnykh regionov severnogo Kazakhstana // Biotekhnologiya. Teoriya i praktika. – 2014. – № 4. (In Russian).

5. Pejkovski Z. Usage of starter cultures as inhibitors of microbiological hazards in fermented meat products. Knowledge / Z. Pejkovski, A. Silovska Nikolova // International Journal. – 2023. – № 58(3). – R. 433-437. Retrieved from https://ikm.mk/ojs/index.php/kij/article/view/6129. (In English).

6. Gänzle M.G. Lactic metabolism revisited: metabolism of lactic acid bacteria in food fermentations and food spoilage // Current Opinion in Food Science. – 2015. – T. 2. – R. 106-117. (In English).

7. García-Díez J. Use of Starter Cultures in Foods from Animal Origin to Improve Their Safety / J. García-Díez, C. Saraiva // International Journal of Environmental Research and Public Health. – 2021. – № 18(5). – R. 2544. https://doi.org/10.3390/ijerph18052544. (In English).

8. Hammes W.P. New developments in meat starter cultures / W.P. Hammes, C. Hertel // Meat Science. –1998. – 49, № 1, S1. (In English).

9. Ponnampalam E.N. Sheep: Meat Reference Module in Food Science / E.N. Ponnampalam, B.W.B. Holman, N.DScollan // in: Encyclopedia of Food and Health. – 2016. – R. 750-757. (In English).

10. Chemical composition and fatty acid content in lamb and adult sheep meat / A. Junkuszew et al // Arch. Anim. Breed. – 2020. – № 63. – R. 261-268. https://doi.org/10.5194/aab-63-261-2020. (In English).

11. Sink J.D. Lamb and mutton flavour: Contributing factors and chemical aspects / J.D. Sink, F. Caporaso // Meat Science. – 1977. – Vol. 1, Issue 2. – P. 119-127. https://doi.org/10.1016/0309-1740(77)90013-4. (In English).

12. Kaz'mina S.V. Obosnovanie ispol'zovaniya startovykh kul'tur v tekhnologii varenokopchenykh kolbas / S.V. Kaz'mina, N.A. Kashirina, E.E. Kurchaeva // Tekhnologii i tovarovedenie sel'skokhozyaistvennoi produktsii. – 2016. – № 1(6). S. 64-72. (In Russian).

13. Two Debaryomyces hansenii strains as starter cultures for improving the nutritional and sensory quality of dry-cured pork belly / Xiaohui Gong et al // Shouwei Wang,Food Research International. – 2024. – Vol. 183. – R. 114227. https://doi.org/10.1016/j.foodres.2024.114227. (In English).

14. Effect of a combination of probiotics on the flavor profiling and biogenic amines of composite fermented mutton sausages / Yana Liu et al // Food Bioscience. – 2024. – Vol. 61. – R. 104835. https://doi.org/10.1016/j.fbio.2024.104835. (In English).

15. Lactic acid bacteria and Staphylococcus carnosus fermentation as a means for eliminating 4-alkyl branched odour fatty acids of mutton jerky and its effect on other quality characteristics / Jiaying Zhou et al // International Journal of Food Science and Technology. – 2022. – Vol. 57, Issue 10. – P. 6843-6851. https://doi.org/10.1111/ijfs.16024. (In English).

16. Physicochemical and microbiological properties of fermented lamb sausages using probiotic Lactobacillus plantarum IIA-2C12 as starter culture / I.I. Arief et al // Proc. Envi. – 2014. – Sci. 20. – R. 352-356. http://dx.doi.org/10.1016/j.proenv.2014.03.044. (In English).

17. A review of selection criteria for starter culture development in the food fermentation industry / G.V.M. Pereira et al // Food Rev. Int. – 2020. – № 36. – R. 135-167. (In English).

18. Laranjo M. Role of Starter Cultures on the Safety of Fermented Meat Products / M. Laranjo, M.E. Potes, M. Elias // Front. Microbiol. – 2019. – № 26. – R. 583. (In English).

19. GOST 32031–2012. Mikrobiologiya pishchevykh produktov i kormov dlya zhivotnykh. Obshchie trebovaniya k metodam vyyavleniya i identifikatsii mikroorganizmov. – Vved. 01.07.2014. – M.: Standartinform, 2014. – 25 s. (In Russian).

20. GOST 10444.11–2013 Mikrobiologiya pishchevykh produktov. Metod podscheta kolichestva mezofil'nykh aehrobnykh i fakul'tativno-anaehrobnykh mikroorganizmov (MAFAM). – Vved. 01.01.2015. – M.: Standartinform, 2014. – 14 s. (In Russian).

21. GOST 34372–2017 – Zakvaski bakterial'nye dlya proizvodstva molochnoi produktsii. Obshchie tekhnicheskie usloviya. – Vved. 01.09.2018. – M.: Standartinform, 2018. – 18 s. (In Russian).

22. GOST 31449-2013 – Produkty pishchevye. Metody vyyavleniya i opredeleniya kolichestva probioticheskikh mikroorganizmov. – Vved. 01.07.2014. – M.: Standartinform, 2019. – 7 s. (In Russian).

23. GOST 23227-2014 – Moloko i molochnye produkty. Metod opredeleniya titruemoi kislotnosti. – Vved. 01.01.2013. – M.: Standartinform, 2019. – 8 s. (In Russian).

24. GOST 31861-2012 Pishchevaya produktsiya. Metod izmereniya pH. – Moloko i molochnye produkty. Metod opredeleniya titruemoi kislotnosti. – Vved. 01.01.2014. – M.: Standartinform, 2019. – 30 s. (In Russian).

25. GOST 31862-2012 Pishchevye produkty. Metod opredeleniya aktivnosti vody. – Vved. 01.01.2014. – M.: Standartinform, 2013. – 7 s. (In Russian).

26. Ponomarev V.Y. Use of a promising strain of Lactobacillus plantarum 131 as a starter culture for fermented sausage production / V.Y. Ponomarev, E.S. Yunusov // AIP Conference Proceedings. – AIP Publishing LLC, 2025. – T. 3268, № 1. – R. 040004. (In English).

27. Perspectives on the probiotic potential of indigenous moulds and yeasts in dry-fermented sausages / M. Álvarez et al // Microorganisms. – 2023. – T. 11, № 7. – R. 1746. (In English).


Review

For citations:


Sharapatova M., Kapshakbayeva Z., Issayeva K. MICROBIAL DIVERSITY OF TRADITIONAL FERMENTED DAIRY PRODUCTS: SPECIES CHARACTERIZATION AND BIOTECHNOLOGICAL PROSPECTS FOR LAMB FERMENTATION. Bulletin of Shakarim University. Technical Sciences. 2025;(3(19)):436-448. (In Russ.) https://doi.org/10.53360/2788-7995-2025-3(19)-49

Views: 681

JATS XML


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 2788-7995 (Print)
ISSN 3006-0524 (Online)
X