THE INFLUENCE OF THE CULTURE MEDIUM COMPOSITION ON THE ESTABLISHMENT OF EXPLANTS OF THE ALMOND LEDEBUROVSKY (AMYGDALUS LEDEBOURIANA SCHLECHT.) IN VITRO
Abstract
The article presents the results of the selection of a culture medium for microclonal propagation of Amygdalus ledebouriana – an endemic of the eastern part of Kazakhstan. The influence of culture media Murashige and Skoog, Gamborg and Eveleigh, and Woody Plant Medium without hormones and supplemented with phytohormones (6-benzylaminopurine, kinetin, thidiazuron) on establishment and growth characteristics of Amygdalus ledebouriana explants in vitro was studied. The composition of the culture medium affects the establishment and growth characteristics (p <0.05) of Amygdalus ledebouriana shoots in vitro culture. MS basal medium was the best culture medium for in vitro propagation of Amygdalus ledebouriana. An increase establishment and growth rates of shoots was achieved by introducing plant growth regulator 6-BAP into the culture medium at a concentration of 1.0 mg/l.
About the Authors
M. Serafimovich
Казахский научно-исследовательский институт лесного хозяйства и агролесомелиорации
Kazakhstan
Kazakhstan
V. Kirillov
Казахский научно-исследовательский институт лесного хозяйства и агролесомелиорации
Kazakhstan
Kazakhstan
T. Stikhareva
Казахский научно-исследовательский институт лесного хозяйства и агролесомелиорации
Kazakhstan
Kazakhstan
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Review
For citations:
Serafimovich M., Kirillov V., Stikhareva T. THE INFLUENCE OF THE CULTURE MEDIUM COMPOSITION ON THE ESTABLISHMENT OF EXPLANTS OF THE ALMOND LEDEBUROVSKY (AMYGDALUS LEDEBOURIANA SCHLECHT.) IN VITRO. Bulletin of Shakarim University. Technical Sciences. 2020;(3(91)):289-293. (In Russ.)
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