ISOLATION AND IDENTIFICATION OF FRIEDELAN-3-ONE FROM THE WILD PLANT RUMEX RECHINGERIANUS LOSINSK

The article presents the results of a study focused on the isolation and structural identification of the triterpenoid compound friedelan-3-one from the wild-growing plant Rumex rechingerianus Losinsk.

The object of the study was the aboveground part of the plant, collected during the growing season in the foothills of the Trans-Ili Alatau (Almaty region, Kazakhstan) in October 2022. The botanical identification was carried out jointly with a specialist from the RSE "Institute of Botany and Phytointroduction" of the National Research University of the Ministry of Education and Science of the Republic of Kazakhstan. The relevance of the work is due to the insufficient knowledge of the chemical composition of this endemic species and the need to expand information on triterpenoid compounds of natural origin, which are of interest to phytochemistry and pharmacognosy.

Extraction of biologically active substances was carried out by maceration using 70% ethanol. The obtained extract was concentrated under reduced pressure and subjected to fractionation by vacuum liquid chromatography using a gradient system of dichloromethane-methanol.

Further separation of the target fractions was performed by column chromatography on silica gel, while the purity of the isolated compounds was monitored by thin-layer chromatography.

Structural identification of the isolated compound was carried out using NMR spectroscopy (¹H, ¹³C, DEPT) and gas chromatography-mass spectrometry. Analysis of the combined spectral data confirmed that the isolated compound belongs to the triterpenoid class and allowed its unambiguous identification as friedelan-3-one. The obtained results supplement existing data on the chemical composition of Rumex rechingerianus Losinsk and provide a basis for further phytochemical and pharmacological studies of this species.

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