JUSTIFICATION OF THE CHOICE OF METHOD AND MATERIAL FOR ENZYME IMMOBILIZATION

The article presents research on the development of a test system for the determination of organophosphate compounds in milk. Based on our research, we selected a hydrolytic enzyme with the highest specific activity and, accordingly, highly sensitive to the inhibitory properties of carbophos in milk, namely, acetylcholinesterase.
The basis for the creation of express methods for the determination of pesticides are physico-chemical, microbiological, biochemical, molecular biological and immunological methods, and they are constantly being improved.
Theoretical and experimental studies were applied in the article.
Experimental studies were conducted on the basis of generally accepted, modified and standard research methods of physico-chemical, organoleptic, rheological, hygienic safety indicators of research objects, as well as the specific activity of enzymes.
Since milk is a complex polydisperse system, the choice of an enzyme with a higher specific activity is an important factor. According to literature data, hydrolytic enzymes (acetylcholinesterases and butyrylcholinesterases) are highly sensitive to the inhibitory properties of carbophos, which is manifested in a decrease in the specific activity of the enzyme.
The results of the experimental studies have shown that of the two hydrolytic enzymes, the highest specific activity is shown by acetylcholinesterase versus butyrylcholinesterase in both aqueous and milk environments.

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