INFLUENCE OF VIRAL INFECTION ON MORPHOMETRIC PARAMETERS TRANSGENIC PLANTS EXPRESSING THE MODIFIED SUPRESSOR P19

The study of morphometric parameters as a result of the impact of viral pathogens on plants is important for determining the resistance of plants to them. In the article, when determining the influence of a viral pathogen on the external parameters of plants, methods for obtaining material that affects plants and actions to damage plants are described. However, the morphological parameters of plants affected by the pathogen were photographed and the results obtained after seven and fourteen days were selected. Changes in morphometric parameters as a result of analysis and analysis between control and transgenic plants from the obtained data are described. The control plant showed that, in contrast to the TR1 and TR2 plants, the morphometric manifestation ceased to exist, and the TR1 plant is resistant to the viral pathogen, since further development of the upper fresh leaves of the TR1 plant was observed. This, in turn, indicates that the P19 protein in transgenic plants provides resistance to THE tbsv virus.

1. Fire A., Xu S., Montgomery M., Kostas S., Driver S., Mello C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditiselegans // Nature : journal, 1998. – № 6669. – P. 806-811.

2. Hammond S., Bernstein E., Beach D., Hannon G. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells // Nature : journal. – 2000. – № 6775. – P. 293-296.

3. Bagasra O., Prilliman K. R.RNA interference: the molecular immune system // J. Mol. Histol., 2004. – № 6. – P. 545–553.

4. Fritz J., Girardin S., Philpott D. Innate immune defense through RNA interference// Sci STKE: journal, 2006. – № 339.– p. 27.

5. Жангазин С. Б. Р19 вирустық ақуыз супрессорының РНҚ-интерференциясын тежеудегі әсерінің молекулалық механизмдері: философия докторы (PhD) ғылым дәрежесін алу үшін дайындалған диссертацияның авторефераты. – Павлодар, 2017.