INTEGRATED SYSTEM OF GEOINFORMATION MONITORING, DIGITAL PASSPORTIZATION, AND SAMPLE PREPARATION OF HALYOMORPHA HALYS STÅL IN SOUTHERN KAZAKHSTAN

The article proposes and tests an integrated technical scheme for the early detection, digital passportization, and preparation of Halyomorpha halys Stål samples for molecular-genetic verification under the conditions of southern Kazakhstan. The aim of the study was to develop a reproducible system combining field monitoring, GPS referencing of foci, standardized recording of samples, and the formation of a collection suitable for subsequent analysis using the COI and ITS1 markers. Field testing of the system was carried out in the Botanical Garden of Almaty using pheromone traps and trap belts on apple and pear trees. During the observations, 215 individuals at different developmental stages were recorded. The highest abundance was detected in the central part of the surveyed territory; the maximum values were recorded at points D1 and D2 (30 and 33 individuals, respectively). It was established that pheromone traps provide more complete detection of adult imagoes, whereas trap belts increase the detectability of nymphal stages. A unified digital passport of the sample was proposed, ensuring the traceability of data from the moment of collection to the stage of laboratory processing and spatial analysis. The developed system can serve as the first stage of dissertation research on the molecular structure of invasive populations of H. halys and as a basis for regional phytosanitary monitoring programs.

1. Leskey T.C. Impact of the invasive brown marmorated stink bug in North America and Europe: History, biology, ecology, and management / T.C. Leskey, A.L. Nielsen // Annual Review of Entomology. – 2018. – Vol. 63. P. 599-618. https://doi.org/10.1146/annurev-ento-020117-043226.

2. Esenbekova P.A. First record of Halyomorpha halys (Stål, 1855) (Heteroptera, Pentatomidae) from Kazakhstan / P.A. Esenbekova // Euroasian Entomological Journal. – 2017. – Vol. 16, № 1. – P. 23-24. https://doi.org/10.15298/euroasentj.16.1.05.

3. Biology, ecology, and management of brown marmorated stink bug (Hemiptera: Pentatomidae) / K.B. Rice et al // Journal of Integrated Pest Management. – 2014. – Vol. 5, № 3. https://doi.org/10.1603/IPM14002.

4. Temreshev I.I. New records of a dangerous invasive pest – brown marmorated stink bug Halyomorpha halys Stål, 1855 (Heteroptera, Pentatomidae) in Kazakhstan / I.I. Temreshev, P.A. Esenbekova, A.M. Uspanov // Acta Biologica Sibirica. – 2018. – Vol. 4, № 3. – P. 94-101. https://doi.org/10.14258/abs.v4i3.4413.

5. Improved trap designs and retention mechanisms for Halyomorpha halys (Hemiptera: Pentatomidae) / K.B. Rice et al // Journal of Economic Entomology. – 2018. – Vol. 111, № 5. – P. 2136-2142. https://doi.org/10.1093/jee/toy185.

6. Halyomorpha halys (Hemiptera: Pentatomidae) responses to traps baited with pheromones in peach and apple orchards / C. Akotsen-Mensah et al // Journal of Economic Entomology. – 2018. – Vol. 111, № 5. – P. 2153-2162. https://doi.org/10.1093/jee/toy200.

7. Characterizing spring emergence of adult Halyomorpha halys using experimental overwintering shelters and commercial pheromone traps / J.C. Bergh et al // Entomologia Experimentalis et Applicata. – 2017. – Vol. 162. – P. 336-345. https://doi.org/10.1111/eea.12539.

8. Monitoring of the invasive Halyomorpha halys, a new key pest of fruit orchards in northern Italy / L. Maistrello et al // Journal of Pest Science. – 2017. – Vol. 90, № 4. – P. 1231-1244. https://doi.org/10.1007/s10340-023-01638-0.

9. Monitoring and biosurveillance tools for the brown marmorated stink bug, Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) / A.L. Acebes-Doria et al // Insects. – 2018. – Vol. 9, № 3, Article 82. https://doi.org/10.3390/insects9030082.

10. Halyomorpha halys (Hemiptera: Pentatomidae) genetic diversity in North America and Europe / D.E. Kapantaidaki et al // Insects. – 2019. – Vol. 10, № 6, Article 174. https://doi.org/10.3390/insects10060174.

11. Real-time PCR assay for the identification of the brown marmorated stink bug (Halyomorpha halys) / M.K. Dhami et al // Frontiers in Molecular Biosciences. – 2016. – Vol. 3, Article 5. https://doi.org/10.3389/fmolb.2016.00005.

12. Seasonal abundance and trap comparisons of the invasive brown marmorated stink bug Halyomorpha halys (Hemiptera: Pentatomidae) adults from its native region / M.T. Kamiyama et al // Biologia. – 2024. – Vol. 79. – P. 1341-1349. https://doi.org/10.1007/s11756-024-01618-4.

13. Diversity and distribution of cytochrome oxidase I (COI) haplotypes of the brown marmorated stink bug, Halyomorpha halys Stål (Hemiptera, Pentatomidae), along the eastern front of its invasive range in Eurasia / T.D. Gariepy et al // NeoBiota. – 2021. – Vol. 68. – P. 53-77. https://doi.org/10.3897/neobiota.68.68915.

14. Review of the biology, ecology, and management of Halyomorpha halys in China, Japan, and Korea / D.-H. Lee et al // Environmental Entomology. – 2013. – Vol. 42, № 4. – P. 627-641. https://doi.org/10.1603/EN13006.