ASSESSMENT OF THE EFFECTIVENESS OF LONG-TERM STORAGE OF MICROORGANISMS CULTURES IN A LYOPHILE-DRIED CONDITION

The development of industrial biotechnology is based on the use of resources from objects such as microbial cultures. Collections of microorganism cultures contain strains that are used in research and applied development. Expanding the resources of industrial microorganisms, optimizing methods for their storage and improving bioproducing properties is an urgent task for the collection of microorganisms. In this work, we studied the maximum viability rate and morpho-cultural characteristics of collection cultures stored for more than 10 years using the freeze-drying method. This was done to evaluate the effectiveness of this storage method and the protective environments used. The object of the study were 32 strains of various taxonomic groups of bacteria, fungi, yeasts and actinomycetes. In sum, we obtained the following results: 2 cultures did not grow after reactivation, 30 cultures corresponded to their passport characteristics and had a viability rate within the acceptable limits for industrial crops, more than 10⁶ CFU/ml: 10⁸-10¹⁰ CFU/ml. The data obtained confirm the effectiveness of storage by lyophilization and the protective media used on gelatin, sucrose and skim milk. Objects of research can be used in various spheres of human life.

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