kaz44Вестник Университета Шакарима. Серия технические наукиBulletin of Shakarim University. Technical Sciences2788-79953006-0524«Шәкәрім университеті» КеАҚ10.53360/2788-7995-2026-1(21)-77kaz44-2338Research ArticleХИМИЧЕСКАЯ ТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)CHEMICAL TECHNOLOGY (ORIGINAL ARTICLE)ОЧИСТКА ВОДЫ МЕТОДОМ ХОЛОДНОЙ ПЛАЗМЫ: ЭКСПЕРИМЕНТАЛЬНАЯ ОЦЕНКА БАКТЕРИЦИДНОГО ДЕЙСТВИЯWATER PURIFICATION BY THE COLD PLASMA METHOD: AN EXPERIMENTAL EVALUATION OF BACTERICIDAL ACTIONhttps://orcid.org/0000-0002-8610-7321ДжумагазиеваА. Б.ZhumagazievaA. B.

Ардак Бисенбаевна Джумагазиева – PhD доктор, заведующий лабораторией микробиологии 

г. Алматы, пр. Аль-фараби, 75Б

Ardak Bissenbayevna Jumagaziyeva – PhD, Head of the Microbiology Laboratory 

050013, Almaty, Satpayev Street, 22А

r_dawa@mail.ruhttps://orcid.org/0000-0001-7428-0074ИскакбаеваЖ. А.IskakbayevaZh. A.

Жанар Алибаевна Искакбаева – заместитель заведующего лабораторией микробиологии 

г. Алматы, пр. Аль-фараби, 75Б

Zhanar Alibayevna Iskakbayeva – Deputy Head of the Microbiology Laboratory 

050013, Almaty, Satpayev Street, 22А

zhanara_07_74@mail.ruhttps://orcid.org/0000-0002-1200-2340ЖантикеевУ. Е.ZhantikeyevU. Ye.

Улан Жантикеев – PhD-докторант кафедры «Материаловедение, нанотехнологии и инженерная физика» 

050013, г. Алматы, ул. Сатпаева, 22

Ulan Yerzhanuly Zhantikeyev – PhD-student of the Department of «Materials Science, Nanotechnology and Engineering Physics» 

050013, Almaty, 22 Satpaeva St.

nurlybekov.ulan@gmail.comhttps://orcid.org/0000-0002-5014-9407БексеитоваК. С.BexeitovaK. S.

Калампыр Сунакбаевна Бексейтова – PhD доктор, научный сотрудник лаборатории инженерного профиля  

050013, г. Алматы, ул. Сатпаева, 22

Kalampyr Sunakbayeva Bexeitova – PhD, researcher of the Laboratory of Engineering Profile 

050013, Almaty, 22 Satpaeva St.

kalampyr.bexeitovas@gmail.comhttps://orcid.org/0000-0002-9705-7438АзатС.AzatS.

Сейтхан Азат – профессор, руководитель лаборатории инженерного профиля 

050013, г. Алматы, ул. Сатпаева, 22

Seitkhan Azat – Professor, Head of the Laboratory of Engineering Profile 

050013, Almaty, 22 Satpaeva St.

s.azat@satbayev.universityЛаборатория микробиологии, АО «Научный центр противоинфекционных препаратов»РоссияLaboratory of Microbiology, JSC «Scientific Center for Anti-Infective Drugs»Russian FederationКазахский национальный исследовательский технический университет им. К.И.СатпаеваКазахстанSatbayev UniversityKazakhstan20262505202611(21)714723Copyright © Джумагазиева А.Б., Искакбаева Ж.А., Жантикеев У.Е., Бексеитова К.С., Азат С., 20262026Джумагазиева А.Б., Искакбаева Ж.А., Жантикеев У.Е., Бексеитова К.С., Азат С.Zhumagazieva A.B., Iskakbayeva Z.A., Zhantikeyev U.Y., Bexeitova K.S., Azat S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://tech.vestnik.shakarim.kz/jour/article/view/2338

Целью данного исследования являлась оценка эффективности установки холодной плазмы для обеззараживания воды, контаминированной бактериальной микрофлорой. Экспериментальная установка CD Plasma генерирует холодную плазму путем ионизации частиц обрабатываемой жидкости, предварительно преобразованной в двухфазную жидко-газовую среду. Холодная плазма оказывает выраженное антимикробное действие за счет синергетического влияния активных форм кислорода и азота, ультрафиолетового излучения и заряженных частиц, которые вызывают окислительное повреждение клеточной стенки, нарушение целостности мембраны, инактивацию белков и фрагментацию ДНК микроорганизмов. В качестве тестобъектов использованы бактерии Escherichia coli и Pseudomonas aeruginosa как представители санитарно-показательной и условно-патогенной микрофлоры. Показано, что воздействие холодной плазмы в течение 5 минут обеспечивает значительное снижение микробной обсеменённости в отношении обеих культур, однако в большей степени в отношении Pseudomonas aeruginosa. Полученные результаты свидетельствуют о высокой эффективности холодной плазмы в инактивации бактерий без использования химических реагентов и указывают на перспективность её применения в технологиях очистки и обеззараживания воды. Метод отличается энергоэффективностью, масштабируемостью и минимальным образованием побочных продуктов при небольших эксплуатационных затратах.

The aim of this study was to assess the effectiveness of a cold plasma unit for the disinfection of water contaminated with bacterial microflora. The CD Plasma experimental setup generates cold plasma by ionizing particles of the treated liquid, which is preliminarily converted into a two-phase liquid – gas medium. Cold plasma exerts a pronounced antimicrobial effect due to the synergistic influence of reactive oxygen and nitrogen species, ultraviolet radiation, and charged particles, which cause oxidative damage to the cell wall, disruption of membrane integrity, inactivation of proteins, and fragmentation of the DNA of microorganisms. As test objects, the bacteria Escherichia coli and Pseudomonas aeruginosa were used as representatives of sanitary-indicator and opportunistic microflora. It was shown that exposure to cold plasma for 5 minutes provides a significant reduction in microbial contamination with respect to both cultures, however to a greater extent with respect to Pseudomonas aeruginosa. The obtained results indicate the high effectiveness of cold plasma in the inactivation of bacteria without the use of chemical reagents and point to the prospects of its application in water treatment and disinfection technologies.

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The authors declare that there are no conflicts of interest present.