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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">kaz44</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Университета Шакарима. Серия технические науки</journal-title><trans-title-group xml:lang="en"><trans-title>Bulletin of Shakarim University. Technical Sciences</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2788-7995</issn><issn pub-type="epub">3006-0524</issn><publisher><publisher-name>«Шәкәрім университеті» КеАҚ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.53360/2788-7995-2026-1(21)-45</article-id><article-id custom-type="elpub" pub-id-type="custom">kaz44-2294</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)</subject></subj-group></article-categories><title-group><article-title>БИОИНДИКАЦИЯ ВЛИЯНИЯ ФОСФОРСОДЕРЖАЩИХ ОТХОДОВ НА ВОДНЫЕ БИОЦЕНОЗЫ РЕКИ ЛЕНГЕР</article-title><trans-title-group xml:lang="en"><trans-title>BIOINDICATION OF THE EFFECT OF PHOSPHORUS-CONTAINING WASTES ON AQUATIC BIOCENOSES OF THE LENGER RIVER</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-3752-7853</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Оспанова</surname><given-names>Ж. Қ.</given-names></name><name name-style="western" xml:lang="en"><surname>Ospanova</surname><given-names>Zh. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Жанна Хожановна Оспанова – кандидат биологических наук, PhD, исполняющая обязанности доцента </p><p>160021, г. Шымкент, ул. Мадели Кожа, 137/1;160031, г. Шымкент, ул. Жибек Жолы, 131</p></bio><bio xml:lang="en"><p>Zhanna Khozhanovna Ospanova – Candidate of Biological Sciences (PhD), Acting Associate Professor </p><p>160021, Shymkent, 137/1 Madeli Kozha St.;160031, Shymkent, 131 Zhibek Zholy St.</p></bio><email xlink:type="simple">zhannaospanova4@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-2081-0935</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Асамбаева</surname><given-names>Л. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Asambaeva</surname><given-names>L. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лаззат Каликбаевна Асамбаева – кандидат биологических наук, старший преподаватель </p><p>160031, г. Шымкент, ул. Жибек Жолы, 131</p></bio><bio xml:lang="en"><p>Lazzat Kalikbaevna Asambaeva – Candidate of Biological Sciences, Senior Lecturer </p><p>160031, Shymkent, 131 Zhibek Zholy St.</p></bio><email xlink:type="simple">asambayeva.lazzat@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9752-5986</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рахимбердиева</surname><given-names>Ж. Ш.</given-names></name><name name-style="western" xml:lang="en"><surname>Rakhimberdieva</surname><given-names>Zh. Sh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Жанар Шерахметовна Рахимбердиева </p><p>160031, г. Шымкент, ул. Жибек Жолы, 131;160018, г. Шымкент, пр. Тауке хана, 5</p></bio><bio xml:lang="en"><p>Zhanar Sherakhmetovna Rakhimberdieva – M. Auezov South Kazakhstan University </p><p>160031, Shymkent, 131 Zhibek Zholy St.;160018, Shymkent, 5 Tauke Khan Ave.</p></bio><email xlink:type="simple">rakhimberdiyeva80@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6865-2003</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Касимбекова</surname><given-names>М. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Kassimbekova</surname><given-names>M. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мереке Даулетбековна Касимбекова – PhD, исполняющая обязанности доцента кафедры технологии фармацевтического производства </p><p>160019, г. Шымкент, пл. Аль-Фараби, 1/1 </p></bio><bio xml:lang="en"><p>Mereke Dauletbekovna Kassimbekova – PhD, Acting Associate Professor of the Department of Pharmaceutical Production Technology </p><p>160019, Shymkent, 1/1 Al-Farabi Square</p></bio><email xlink:type="simple">k.m.dauletbekovna@gmail.com</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Центрально-Азиатский инновационный университет;&#13;
Шымкентский университет</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Central Asian Innovation University;&#13;
Shymkent University</institution><country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Шымкентский университет</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Shymkent University</institution><country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Шымкентский университет;&#13;
Южно-Казахстанский университет имени М. Ауэзова</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>Shymkent University;&#13;
M. Auezov South Kazakhstan University</institution><country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Южно-Казахстанская медицинская академия</institution><country>Казахстан</country></aff><aff xml:lang="en"><institution>South Kazakhstan Medical Academy</institution><country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>25</day><month>05</month><year>2026</year></pub-date><volume>1</volume><issue>1(21)</issue><fpage>406</fpage><lpage>415</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Оспанова Ж.Қ., Асамбаева Л.К., Рахимбердиева Ж.Ш., Касимбекова М.Д., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Оспанова Ж.Қ., Асамбаева Л.К., Рахимбердиева Ж.Ш., Касимбекова М.Д.</copyright-holder><copyright-holder xml:lang="en">Ospanova Z.K., Asambaeva L.K., Rakhimberdieva Z.S., Kassimbekova M.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://tech.vestnik.shakarim.kz/jour/article/view/2294">https://tech.vestnik.shakarim.kz/jour/article/view/2294</self-uri><abstract><p>В статье исследовано влияние фосфорсодержащих отходов техногенного происхождения на экологическое состояние водной экосистемы реки Ленгер, расположенной в южном регионе Казахстана. Отбор проб воды осуществлялся в июне 2025 года на пяти участках, характеризующихся различным уровнем антропогенной нагрузки. Лабораторные эксперименты проводились с использованием образцов речной воды и техногенных породных отвалов с добавлением фосфорсодержащих остатков в концентрациях 0,1%, 0,3%, 0,5% и 1,0%; контрольные образцы не содержали дополнительных примесей. Инкубация осуществлялась в течение 72 часов при температуре 22-24 °C в условиях естественного освещения. В ходе таксономического анализа были выявлены представители фитопланктона и простейших, относящиеся к отделам Chlorophyta, Euglenophyta, Bacillariophyta, Cyanophyta и Protozoa. Среди зелёных водорослей доминировали Chlorella vulgaris и Scenedesmus quadricauda; диатомовые водоросли были представлены преимущественно видами Navicula vulpina и Fragillaria austriaca; эвгленовые водоросли – Euglena agilis и Phacus brevicaudatus. В пробах также отмечено присутствие цианопрокариот (Oscillatoria, Anabaena) и простейших (Paramecium, Arcella, Euplotes), что свидетельствует о высоком трофическом статусе водоёма и наличии органического загрязнения. При концентрациях 0,1-0,3% наблюдалось активное развитие Chlorella vulgaris и Scenedesmus quadricauda, тогда как при 0,5-1,0% отмечались угнетение роста большинства видов, снижение прозрачности воды и формирование органо-минеральных осадков. Наибольшую чувствительность к фосфатному загрязнению проявили диатомовые водоросли. Полученные результаты показывают, что при низких концентрациях фосфорсодержащих отходов фотосинтетическая активность микроводорослей возрастает, что отражает их способность использовать поступающие вещества в качестве источников питания. Однако повышение концентраций до пороговых значений приводит к нарушению устойчивости водных сообществ и деградации биоценоза. Таким образом, выявленные закономерности подтверждают возможность использования микроводорослей и простейших в качестве надёжных биоиндикаторов антропогенного воздействия и экологического состояния малых речных экосистем Казахстана.</p></abstract><trans-abstract xml:lang="en"><p>This article investigates the impact of technogenic phosphorus-containing waste on the ecological condition of the aquatic ecosystem of the Lenger River, located in the southern region of Kazakhstan. Water samples were collected in June 2025 from five sites characterized by different levels of anthropogenic load. Laboratory experiments were conducted using river water samples and technogenic waste rock materials with the addition of phosphorus-containing residues at concentrations of 0.1%, 0.3%, 0.5%, and 1.0%; control samples did not contain any additional impurities. Incubation was carried out for 72 hours at a temperature of 22-24°C under natural light conditions. Taxonomic analysis revealed representatives of phytoplankton and protozoa belonging to the divisions Chlorophyta, Euglenophyta, Bacillariophyta, Cyanophyta, and Protozoa. Among green algae, Chlorella vulgaris and Scenedesmus quadricauda predominated; diatoms were represented mainly by Navicula vulpina and Fragillaria austriaca; euglenophytes included Euglena agilis and Phacus brevicaudatus. Samples also contained cyanoprokaryotes (Oscillatoria, Anabaena) and protozoa (Paramecium, Arcella, Euplotes), indicating high trophic status and organic pollution of the water. At concentrations of 0.1-0.3%, active development of Chlorella vulgaris and Scenedesmus quadricauda was observed, whereas at 0.5-1.0% most species exhibited growth inhibition, decreased water transparency, and the formation of organo-mineral sediments. Diatom algae showed the highest sensitivity to phosphate contamination. The obtained results demonstrate that low concentrations of phosphorus-containing wastes enhance the photosynthetic activity of microalgae, indicating their ability to utilize these substances as nutrient sources. However, increasing concentrations to threshold levels lead to destabilization of aquatic communities and degradation of the biocenosis. Thus, the revealed patterns confirm that microalgae and protozoa can be effectively used as reliable bioindicators of anthropogenic impact and ecological status in small river ecosystems of Kazakhstan.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>река Ленгер</kwd><kwd>фосфорсодержащие отходы</kwd><kwd>микроводоросли</kwd><kwd>простейшие</kwd><kwd>биоиндикация</kwd><kwd>сапробность</kwd><kwd>экологическое состояние</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Lenger River</kwd><kwd>phosphorus-containing wastes</kwd><kwd>microalgae</kwd><kwd>protozoa</kwd><kwd>bioindication</kwd><kwd>saprobity</kwd><kwd>ecological status</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Eutrophication: Causes, Consequencesand Controlsin Aquatic Ecosystems. Nature Education Knowledge Project (Scitable). 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