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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">kaz44</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Университета Шакарима. Серия технические науки</journal-title><trans-title-group xml:lang="en"><trans-title>Bulletin of Shakarim University. Technical Sciences</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2788-7995</issn><issn pub-type="epub">3006-0524</issn><publisher><publisher-name>«Шәкәрім университеті» КеАҚ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.53360/2788-7995-2025-4(20)-44</article-id><article-id custom-type="elpub" pub-id-type="custom">kaz44-2163</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)</subject></subj-group></article-categories><title-group><article-title>ИСПОЛЬЗОВАНИЕ БИОЛОГИЧЕСКИ АКТИВНЫХ КОМПОНЕНТОВ ШПИНАТА ОГОРОДНОГО В БИОТЕХНОЛОГИИ: СОВРЕМЕННЫЕ ТЕНДЕНЦИИ В ОБЛАСТИ СОЗДАНИЯ НАТУРАЛЬНОЙ РАСТИТЕЛЬНОЙ ПРОДУКЦИИ</article-title><trans-title-group xml:lang="en"><trans-title>APPLICATION OF BIOACTIVE COMPOUNDS FROM GARDEN SPINACH IN BIOTECHNOLOGY: CURRENT TRENDS IN THE DEVELOPMENT OF NATURAL PLANT-BASED PRODUCTS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0252-3767</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Жаппарбергенова</surname><given-names>Э. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Zhapparbergenova</surname><given-names>E. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Эльмира Бегимбаевна Жаппарбергенова – кандидат биологических наук, доцент, преподаватель кафедры биологии</p><p>160000, Республика Казахстан, г. Шымкент. ул. Байтурсынова 13 </p></bio><bio xml:lang="en"><p>Elmira Begimbaevna Zhapparbergenova – Candidate of Biological Sciences, Associate Professor, Lecturer of the Department of Biology</p><p>160000, Republic of Kazakhstan, Shymkent, Baitursynov Street, 13 </p></bio><email xlink:type="simple">zhaffarelmira@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1533-0366</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алпамысова</surname><given-names>Г. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Alpamysova</surname><given-names>G. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Гульжайна Байгонысовна Алпамысова – кандидат сельскохозяйственных наук, доцент, заведующая кафедрой биотехнологии</p><p>160050, Республика Казахстан г. Шымкент, пр.Тауке хана 5</p></bio><bio xml:lang="en"><p>Gulzhayna Baigonysovna Alpamysova – Candidate of Agricultural Sciences, Associate Professor, Head of the Department of Biotechnology</p><p>160050, Republic of Kazakhstan, Shymkent, Tauke Khan Avenue, 5 </p></bio><email xlink:type="simple">xap68@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Южно-Казахстанский педагогический университет имени Ө.Жәнібека<country>Казахстан</country></aff><aff xml:lang="en">South Kazakhstan Pedagogical University named after Zhanibekov<country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Южно-Казахстанский университет имени М. Ауэзова<country>Казахстан</country></aff><aff xml:lang="en">South Kazakhstan University named after M. Auezov<country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>25</day><month>01</month><year>2026</year></pub-date><volume>1</volume><issue>4(20)</issue><fpage>368</fpage><lpage>379</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Жаппарбергенова Э.Б., Алпамысова Г.Б., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Жаппарбергенова Э.Б., Алпамысова Г.Б.</copyright-holder><copyright-holder xml:lang="en">Zhapparbergenova E.B., Alpamysova G.B.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://tech.vestnik.shakarim.kz/jour/article/view/2163">https://tech.vestnik.shakarim.kz/jour/article/view/2163</self-uri><abstract><p>Научные исследования посвящены процессам культивации Шпината огородного (Spinacia oleracea) и дальнейшим этапам приготовления из выращенного растения вакуумированного порошка (БАД) и экомыла. Основной объект исследования – Шпинат огородный на сегодняшний день является одним из общепризнанных лидеров овощной зелени в рационе современного человека. Данный факт связан с наличием широкого спектра биологически важных химических компонентов в данном растении. В связи с этим в рамках данного исследования была поставлена задача создания современного продукта с длительным сроком хранения, удобным в ежедневном применении, сохраняющим полноценный биохимический состав, доступный с экономической точки зрения. Немаловажным аспектом эксперимента был контроль условий культивации, не допускающий использование химических удобрений, поскольку шпинат обладает свойством накапливать эти вещества в биомассе в больших количествах. Процесс культивации зеленой биомассы сопровождался мониторингом показателей температуры, влажности, освещения и частоты полива, что позволило к концу культивирования провести сравнительный анализ в динамике роста в разрезе времени.Исследования, направленные на создание экспериментальной биотехнологической продукции – вакуумированного порошка из высушенных листьев шпината, позволили получить биологически активную пищевую добавку длительного хранения (в течении одного года). Предлагаемая добавка, являющаяся альтернативой свежему шпинату, обладает преимуществом внесезонной продукции, полноценно сохраняющей биологическую активность в вакуумированном состоянии. Также важен спектр возможностей применения порошка шпината как в традиционной рецептуре, так и в составе коктейлей и смузи, и как натурального красителя в кондитерскойй технологии. С целью сравнительного анализа сохранения биологической активности в вакуумированном порошке и в свежесобранных листьях шпината, был проведен хроматографический анализ. Индикатором биологической активности явились хлорофиллы a и b, содержащиеся в свежих и высушенных листьях шпината.На заключительном этапе исследований была разработана технология использования свежевыжатого сока молодого шпината, так называемого «baby spinach», для изготовления экомыла. Урожай «baby spinach» собирался на второй неделе культивации в заданных условиях, затем был собран и измельчен путем жидкостной экстракции. В традиционный технологический процесс варки мыльной массы был включен этап внедрения жидкой растительной биомассы (экстракта шпината).</p></abstract><trans-abstract xml:lang="en"><p>Scientific research is devoted to the processes of cultivation of garden Spinach (Spinacia oleracea) and further stages of preparation of evacuated powder (dietary supplements) and eco-soap from the grown plant. The main object of research is Garden spinach, which is currently one of the generally recognized leaders of vegetable greens in the diet of modern humans. This fact is due to the presence of a wide range of biologically important chemical components in this plant. In this regard, within the framework of this study, the task was set to create a modern product with a long shelf life, convenient for daily use, preserving a fullfledged biochemical composition, affordable from an economic point of view. An important aspect of the experiment was the control of cultivation conditions, which does not allow the use of chemical fertilizers, since spinach has the property of accumulating these substances in biomass in large quantities. The process of green biomass cultivation was accompanied by monitoring of temperature, humidity, lighting, and watering frequency, which allowed for a comparative analysis of growth dynamics over time by the end of cultivation.Research aimed at creating experimental biotechnological products – evacuated powder from dried spinach leaves, allowed us to obtain a biologically active food additive for long-term storage (for one year). The proposed additive, which is an alternative to fresh spinach, has the advantage of off-season products that fully preserve biological activity in a vacuum state. The range of spinach powder applications is also important, both in the traditional formulation, as well as in cocktails and smoothies, and as a natural colorant in confectionery technology. In order to compare the preservation of biological activity in the evacuated powder and in freshly harvested spinach leaves, a chromatographic analysis was performed. The indicator of biological activity was chlorophylls a and b contained in fresh and dried spinach leaves.At the final stage of the research, a technology was developed for using freshly squeezed young spinach juice, the so-called «baby spinach», to make eco-soap. The «baby spinach» crop was harvested in the second week of cultivation under the specified conditions, then it was harvested and crushed by liquid extraction. The stage of introduction of liquid plant biomass (spinach extract) was included in the traditional technological process of cooking soap mass.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>биологически активные добавки</kwd><kwd>растительная биомасса</kwd><kwd>процесс культивации</kwd><kwd>вакуумированный порошок</kwd><kwd>экомыло</kwd></kwd-group><kwd-group xml:lang="en"><kwd>biologically active additives</kwd><kwd>plant biomass</kwd><kwd>cultivation process</kwd><kwd>powder vacuumization</kwd><kwd>eco-soap</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Вековцев А. Инновационная технология БАД: характеристика потребительских свойств / А. Вековцев, Е. Ермолаева, Г. Подзорова // Пищевая промышленность. – 2010. – № 4. – С. 52.</mixed-citation><mixed-citation xml:lang="en">Vekovtsev A. Innovatsionnaya tekhnologiya BAD: kharakteristika potrebitel'skikh svoistv / A. 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