kaz44Вестник Университета Шакарима. Серия технические наукиBulletin of Shakarim University. Technical Sciences2788-79953006-0524«Шәкәрім университеті» КеАҚ10.53360/2788-7995-2025-2(18)-20kaz44-1700Research ArticleПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)СОСТАВ МИКРОФЛОРЫ ОТХОДОВ ЛЕНГЕРСКОГО БУРОГО УГЛЯCOMPOSITION OF MICROFLORA OF LENGER BROWN COAL WASTEhttps://orcid.org/0009-0005-0237-158XАлиханА. Ж.AlikhanA.

Акмарал Жапбарқызы Алихан – докторант 3-го курса кафедры «Биотехнология»,

160000, Шымкент, Тауке-хан 5

Akmaral Alikhan – 3rd year doctoral student of the Department of Biotechnology,

160000, Shymkent, Tauke Khan 5

akmaralka_050393@mail.ruhttps://orcid.org/0000-0001-8323-3982ИсаеваА. У.IssayevaA.

Акмарал Умирбековна Исаева – профессор кафедры «Биотехнология», 160000, Шымкент, Тауке-хан 5;

профессор, д.б.н., директор НИИ «Биология и экология», проректор по научной работе, Шымкент, Каратауский р/н, квартал 225, зд. 426

Akmaral Issayeva – professor of the Department of Biotechnology, 160000, Shymkent, Tauke Khan 5;

professor, Doctor of Biological Sciences, Director of the Research Institute of Biology and Ecology, Vice-Rector for Research, 160012 Shymkent, Karatau district, block 225, building 426

 

akissayeva_@mail.ruhttps://orcid.org/0000-0002-8530-3443КудасоваД.KudasovaD.

Дариха Ерадиловна Кудасова – преподаватель кафедры «Биотехнология», 

160000, Шымкент, Тауке-хан 5

Darikha Kudasova – lecturer of the Department of Biotechnology, 

160000, Shymkent, Tauke Khan 5

darikha.kudasova@mail.ruhttps://orcid.org/0000-0001-9752-5986РахымбердиеваЖ.RakhymberdievaZh.

Жанар Шерахметовна Рахымбердиева – доцент кафедры «Биология и География», 160000, Шымкент, Тауке-хан 5;

директор НИИ «Педагогика и психология», Шымкент, Каратауский р/н, квартал 225, зд. 426

Zhanar Rakhymberdieva – PhD, Associate Professor, Department of Biology and Geography, 160000, Shymkent, Tauke Khan 5;

Director of the Research Institute of Pedagogy and Psychology, 160012 Shymkent, Karatau district, block 225, building 426

rakhimberdiyeva_80@mail.ruhttps://orcid.org/0009-0008-5119-8949РысбаеваЖ.RysbaevaZh.

Жанар Алтынбековна Рысбаева – докторант 2-го курса кафедры «Биотехнология», 

160000, Шымкент, Тауке-хан 5

Zhanar Rysbaeva – 2 year doctoral student of the Department of Biotechnology, 

160000, Shymkent, Tauke Khan 5

rysbaeva_janar87@mail.ruЮжно-Казахстанский университет имени М. АуэзоваКазахстанM. Auezov South Kazakhstan UniversityKazakhstanЮжно-Казахстанский университет имени М. Ауэзова; Шымкентский университетКазахстанM. Auezov South Kazakhstan University; Shymkent UniversityKazakhstan20250807202502(18)166176Copyright © Алихан А.Ж., Исаева А.У., Кудасова Д., Рахымбердиева Ж., Рысбаева Ж., 20252025Алихан А.Ж., Исаева А.У., Кудасова Д., Рахымбердиева Ж., Рысбаева Ж.Alikhan A., Issayeva A., Kudasova D., Rakhymberdieva Z., Rysbaeva Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://tech.vestnik.shakarim.kz/jour/article/view/1700

В данной статье проведены генетический анализ видовой идентификации состава естественной микрофлоры отходов буроугольного производства Ленгерского месторождения Туркестанской области и ПЦР-анализ чистых штаммов микроорганизмов. Понимание экологического значения микроорганизмов в отходах бурого угля позволяет оценить их влияние на окружающую среду, сельское хозяйство и процессы производства энергии. Установлено, что в состав микрофлоры отходов бурого угля входят целлюлозоразрушающие и гетеротрофные бактерии, микромицеты и актиномицеты. Основной видовой состав был представлен из микромицетов представителями родов Mucorales, Aspergillus, Fusarium, Candida, из бактерий – представителями родов Bacillus, Rodococcus, Pseudomonas, а также представителями азотфиксирующих бактерий. В результате молекулярно-генетического анализа с целью идентификации штаммов бактерий методом секвенирования по Сэнгеру были выявлены: штамм LB.1 – Priestia megaterium, штамм LA.8 – Pseudomonas sp., штамм LB.M2 – Aspergillus sydowii. Данное исследование позволяет глубоко понять роль полезных микроорганизмов в промышленных отходах бурых углей в экологических процессах, их значимость в природе и на производстве. Полученные результаты исследований помогают определить пути эффективного использования микроорганизмов в отходах бурого угля для повышения плодородия почвы или использования в биотехнологических целях.

This article presents a genetic analysis of the species identification of the natural microflora composition of brown coal waste from the Lenger deposit in the Turkestan region and a PCR analysis of pure microorganism strains. Understanding the ecological significance of microorganisms in brown coal waste allows us to assess their impact on the environment, agriculture and energy production processes. It has been established that the microflora of brown coal waste includes cellulose-destroying and heterotrophic bacteria, micromycetes and actinomycetes. The main species composition was represented by micromycetes from the genera Mucorales, Aspergillus, Fusarium, Candida, and bacteria from the genera Bacillus, Rodococcus, Pseudomonas, as well as nitrogen-fixing bacteria. As a result of molecular genetic analysis to identify bacterial strains using the Sanger sequencing method, the following were identified: LB.1 strain – Priestia megaterium, LA.8 strain – Pseudomonas sp., LB.M2 strain – Aspergillus sydowii. This research allows to deeply understand the role of beneficial microorganisms in industrial wastes of brown coal in ecological processes, their significance in nature and in production. The obtained research results help to determine the way of effective use of microorganisms in brown coal waste to increase soil fertility or use for biotechnological purposes.

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The authors declare that there are no conflicts of interest present.