<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">kaz44</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Университета Шакарима. Серия технические науки</journal-title><trans-title-group xml:lang="en"><trans-title>Bulletin of Shakarim University. Technical Sciences</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2788-7995</issn><issn pub-type="epub">3006-0524</issn><publisher><publisher-name>«Шәкәрім университеті» КеАҚ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.53360/2788-7995-2025-1(17)-38</article-id><article-id custom-type="elpub" pub-id-type="custom">kaz44-1676</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)</subject></subj-group></article-categories><title-group><article-title>ТЕХНОЛОГИЯ ПРОИЗВОДСТВА ГРЕЧЕСКОГО ЙОГУРТА И ОПРЕДЕЛЕНИЕ  В ЕГО СЫВОРОТКЕ КОЛИЧЕСТВА БЕЛКОВ</article-title><trans-title-group xml:lang="en"><trans-title>TECHNOLOGY FOR MAKING GREEK YOGURT AND DETERMINING  THE AMOUNT OF PROTEIN IN ITS WHEY</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2658-9514</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Толеубекова</surname><given-names>С. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Toleubekova</surname><given-names>S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сандугаш Сайлауовна Толеубекова – кандидат технических наук, ассоциированный профессор; кафедра «Биотехнология»</p><p>071412, город Семей, ул. Глинки 20А</p></bio><bio xml:lang="en"><p>Sandugash Toleubekova – Candidate of Technical Sciences, Associate Professor; Department of Biotechnology</p><p>071412, Semey city, Glinka street 20A</p></bio><email xlink:type="simple">saltosha-sandu@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0003-9694-585X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Әліпов</surname><given-names>Ә. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Alipov</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Әділ Ерболұлы Әліпов – магистрант 2 курса ОП 7М07501 «Стандартизация и сертификация»</p><p>071412, город Семей, ул. Глинки 20А</p></bio><bio xml:lang="en"><p>Adil Alipov – master of the 2nd year of the specialty «Standardization and certification»</p><p>071412, Semey city, Glinka street 20A</p></bio><email xlink:type="simple">lpov.dl@bk.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9707-0539</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Жарыкбасов</surname><given-names>Е. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Zharykbasov</surname><given-names>Ye.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ерлан Сауыкович Жарыкбасов – PhD, кафедра «Биотехнология»</p><p>071412, город Семей, ул. Глинки 20А</p></bio><bio xml:lang="en"><p>Yerlan Zharykbasov – PhD; Department of Biotechnology</p><p>071412, Semey city, Glinka street 20A</p></bio><email xlink:type="simple">erlan-0975@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9169-6722</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Джумажанова</surname><given-names>М. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Jumazhanova</surname><given-names>M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мадина Муратовна Джумажанова – PhD, кафедра «Биотехнология»</p><p>071412, город Семей, ул. Глинки 20А</p></bio><bio xml:lang="en"><p>Madina Jumazhanova – PhD; Department of Biotechnology</p><p>071412, Semey city, Glinka street 20A</p></bio><email xlink:type="simple">madina.omarova.89@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-6558-1868</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Қабденова</surname><given-names>А. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Kabdenova</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Айнур Толеухановна Қабденова – магистр биотехнологии; кафедра «Биотехнология»</p><p>071412, город Семей, ул. Глинки 20А</p></bio><bio xml:lang="en"><p> Ainur Kabdenova – Master of Biotechnology; Department of Biotechnology</p><p>071412, Semey city, Glinka street 20A</p></bio><email xlink:type="simple">ain_arik@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Университет имени Шакарима города Семей<country>Казахстан</country></aff><aff xml:lang="en">Shakarim University of Semey<country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>29</day><month>03</month><year>2025</year></pub-date><volume>0</volume><issue>1(17)</issue><fpage>298</fpage><lpage>304</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Толеубекова С.С., Әліпов Ә.Е., Жарыкбасов Е.С., Джумажанова М.М., Қабденова А.Т., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Толеубекова С.С., Әліпов Ә.Е., Жарыкбасов Е.С., Джумажанова М.М., Қабденова А.Т.</copyright-holder><copyright-holder xml:lang="en">Toleubekova S., Alipov A., Zharykbasov Y., Jumazhanova M., Kabdenova A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://tech.vestnik.shakarim.kz/jour/article/view/1676">https://tech.vestnik.shakarim.kz/jour/article/view/1676</self-uri><abstract><p> В данной научной статье рассматривается технология производства греческого йогурта с использованием обезжиренного молока и добавлением жира со средней длиной цепи триглицерида (МСТ). Особое внимание уделено анализу содержания белка в сыворотке, полученной после удаления лишней жидкости из йогурта. Цель исследования – определить количество белка в сыворотке и оценить возможности его дальнейшего использования для обогащения готовых продуктов. Процесс определения белка осуществляли методом Брэдфорда с использованием спектрофотометра, который позволял количественно анализировать количество белка в оставшейся сыворотке. Метод Брэдфорда основан на взаимодействии белков с красителем кумасси Diamond Blue G-250, который связывается с белками и изменяет цвет раствора, что позволяет измерять концентрацию белка по оптической плотности. Результаты исследований показывают, что сывороточные белки имеют высокую возможность повторного использования. Обогащение греческого йогурта этими белками может повысить его пищевую ценность за счет увеличения содержания белка. Таким образом, предложен инновационный способ использования вторичного продукта производства греческого йогурта, позволяющий повысить эффективность производственного процесса и сократить пищевые отходы. Результаты могут быть полезны для предприятий молочной промышленности, занимающихся производством кисломолочных продуктов, а также для научных исследований в области технологии пищевых продуктов и питания. Использование данного метода анализа и обработки сывороточных белков способствует созданию продуктов высокой биологической ценности и снижению себестоимости производства. Таким образом, предлагаемая технология является перспективным направлением для дальнейших исследований и внедрения в промышленное производство.</p></abstract><trans-abstract xml:lang="en"><p>This scientific article discusses the technology for the production of Greek yogurt using skim milk and adding medium-chain triglyceride (MCT) fat. The emphasis is on the analysis of the protein content of the whey obtained after removing excess liquid from the yogurt. The purpose of the study is to determine the amount of whey protein and assess the possibilities of its further use for enriching the finished product. The protein determination process was carried out by the Bradford method using a spectrophotometer, which made it possible to quantitatively analyze the amount of protein in the remaining serum. Bradford's method is based on the interaction of proteins with the kumassi dye Diamond Blue G-250, which binds to proteins and changes the color of the solution, which makes it possible to measure protein concentration by optical density. The results of the study show that whey proteins have a high potential for reuse. Enriching Greek yogurt with these proteins can increase its nutritional value by increasing the protein content. Thus, an innovative approach is proposed to use the by-product of the production of Greek yogurt, which helps to increase the efficiency of the production process and reduce food waste. The results can be useful for dairy industry enterprises engaged in the production of fermented milk products, as well as for scientific research in food technology and nutritiology. The use of this method of analysis and processing of whey proteins contributes to the creation of products of high biological value and reducing production costs. Thus, the proposed technology is a promising direction for further research and implementation in industrial production. </p></trans-abstract><kwd-group xml:lang="ru"><kwd>греческий йогурт</kwd><kwd>сывороточные белки</kwd><kwd>метод Брэдфорда</kwd><kwd>спектрофотометрия</kwd><kwd>масло МСТ</kwd><kwd>анализ белка</kwd><kwd>функциональное питание</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Greek yogurt</kwd><kwd>whey proteins</kwd><kwd>Bradford method</kwd><kwd>spectrophotometry</kwd><kwd>MST oil</kwd><kwd>protein analysis</kwd><kwd>functional food</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Кайшев В.Г. Состояние и перспективы развития рынка функциональных продуктов питания / В.Г. Кайшев, С.Н. Сергин // Переработка молока. – 2018. – № 2. – С. 64-67.</mixed-citation><mixed-citation xml:lang="en">Kaishev V.G. Sostoyanie i perspektivy razvitiya rynka funktsional'nykh produktov pitaniya / V.G. Kaishev, S.N. Sergin // Pererabotka moloka. – 2018. – № 2. – S. 64-67. (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Onecia B. Chronic Pancreatitis / B. Onecia, L.L. Sarah. – 2022. https://www.ncbi.nlm.nih.gov/books/NBK482325/.</mixed-citation><mixed-citation xml:lang="en">Onecia B. Chronic Pancreatitis / B. Onecia, L.L. Sarah. – 2022. https://www.ncbi.nlm.nih.gov/books/NBK482325/. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Петров В.Н. Хронический холесцестит / В.Н. Петров, В.А. Лапотников // Санкт-Петербургская медицинская академия последипломного образования, Санкт-Петербургский государственный медицинский университет им. акад. Павлова. – 2011.</mixed-citation><mixed-citation xml:lang="en">Petrov V.N. Khronicheskii kholestsestit / V.N. Petrov, V.A. Lapotnikov // Sankt-Peterburgskaya meditsinskaya akademiya poslediplomnogo obrazovaniya, Sankt-Peterburgskii gosudarstvennyi meditsinskii universitet im. akad. Pavlova. – 2011. (In Russian).</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Nutrition Guidelines for Chronic Pancreatitis Patient Education // Digestive Health Center at Stanford Hospital and Clinics // NS 12/2012.</mixed-citation><mixed-citation xml:lang="en">Nutrition Guidelines for Chronic Pancreatitis Patient Education // Digestive Health Center at Stanford Hospital and Clinics // NS 12/2012. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Chapman-Lopez T.J. The Effects of Medium-Chain Triglyceride Oil Supplementation on Endurance Performance and Substrate Utilization in Healthy Populations: A Systematic Review / T.J. Chapman-Lopez, Yu. Koh // PMCID: PMC9579472 PMID: 36096496. – 2022. – № 31(3). – Р. 217-229.</mixed-citation><mixed-citation xml:lang="en">Chapman-Lopez T.J. The Effects of Medium-Chain Triglyceride Oil Supplementation on Endurance Performance and Substrate Utilization in Healthy Populations: A Systematic Review / T.J. Chapman-Lopez, Yu. Koh // PMCID: PMC9579472 PMID: 36096496. – 2022. – № 31(3). – R. 217-229. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Medium-Chain Triglyceride Oil and Blood Lipids: A Systematic Review and Meta-Analysis of Randomized Trials / K.M. McKenzie et al // The Journal of Nutrition. – 2021. – Vol. 151, Issue 10. – P. 2949-2956.</mixed-citation><mixed-citation xml:lang="en">Medium-Chain Triglyceride Oil and Blood Lipids: A Systematic Review and Meta-Analysis of Randomized Trials / K.M. McKenzie et al // The Journal of Nutrition. – 2021. – Vol. 151, Issue 10. – P. 2949-2956. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Separation Technologies for Whey Protein Fractionation / G.Q. Chen et al. 2023. – Vol. 15. – Р. 438-465.</mixed-citation><mixed-citation xml:lang="en">Separation Technologies for Whey Protein Fractionation / G.Q. Chen et al. 2023. – Vol. 15. – R. 438-465. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Ganju S. A review on approaches for efficient recovery of whey proteins from dairy industry effluents / S. Ganju, PR. Gogate // J Food Eng. – 215. – Р. 84-96.</mixed-citation><mixed-citation xml:lang="en">Ganju S. A review on approaches for efficient recovery of whey proteins from dairy industry effluents / S. Ganju, PR. Gogate // J Food Eng. – 215. – R. 84-96. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Membrane processes for whey proteins separation and purification a review / R. Aguero et al // Curr Org Chem. – № 21(17). – Р. 1740-1752.</mixed-citation><mixed-citation xml:lang="en">Membrane processes for whey proteins separation and purification a review / R. Aguero et al // Curr Org Chem. – № 21(17). – R. 1740-1752. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Zydney AL. Protein separations using membrane filtration: new opportunities for whey fractionation / AL. Zydney // Int Dairy J. – № 8(3). – Р. 243-250. https://doi.org/10.1016/s09586946(98)00045-4.</mixed-citation><mixed-citation xml:lang="en">Zydney AL. Protein separations using membrane filtration: new opportunities for whey fractionation / AL. Zydney // Int Dairy J. – № 8(3). – R. 243-250. https://doi.org/10.1016/s09586946(98)00045-4. (In English).</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru"></mixed-citation><mixed-citation xml:lang="en"></mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
