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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">kaz44</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Университета Шакарима. Серия технические науки</journal-title><trans-title-group xml:lang="en"><trans-title>Bulletin of Shakarim University. Technical Sciences</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2788-7995</issn><issn pub-type="epub">3006-0524</issn><publisher><publisher-name>«Шәкәрім университеті» КеАҚ</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.53360/2788-7995-2024-3(15)-27</article-id><article-id custom-type="elpub" pub-id-type="custom">kaz44-1275</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)</subject></subj-group></article-categories><title-group><article-title>ВАЛОРИЗАЦИЯ ЛИГНОЦЕЛЛЮЛОЗНОЙ БИОМАССЫ С ПОМОЩЬЮ ТЕМНОВОЙ ФЕРМЕНТАЦИИ: ВЫРАБОТКА ЭФФЕКТИВНЫХ УСЛОВИЙ БИОСИНТЕЗА МОЛЕКУЛЯРНОГО ВОДОРОДА</article-title><trans-title-group xml:lang="en"><trans-title>VALORIZATION OF LIGNOCELLULOSIC BIOMASS BY DARK FERMENTATION: DEVELOPMENT OF EFFECTIVE CONDITIONS FOR MOLECULAR HYDROGEN BIOSYNTHESIS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9591-0370</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Бекбаев</surname><given-names>К. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Bekbayev</surname><given-names>K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кайрат Серикжанович Бекбаев – кандидат технических наук, старший преподаватель кафедры «Технология пищевых и перерабатывающих производств»,</p><p>010000, Aстана, проспект Жеңіс, 62</p></bio><bio xml:lang="en"><p>Kairat Bekbayev – candidate of technical sciences, senior lecturer of the department «Technology of food and processing industries»,</p><p>010000, Astana, Zhenis avenue, 62</p></bio><email xlink:type="simple">k_bekbaev@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-2061-1699</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Tөлеуғазықызы</surname><given-names>А.</given-names></name><name name-style="western" xml:lang="en"><surname>Toleugazykyzy</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ақерке Төлеуғазықызы – PhD, преподаватель кафедры «Технология пищевых и перерабатывающих производств»; </p><p>010000, Aстана, проспект Жеңіс, 62</p></bio><bio xml:lang="en"><p>Akerke Toleugazykyzy – PhD, lecturer of the department «Technology of food and processing industries»,</p><p>010000, Astana, Zhenis avenue, 62</p></bio><email xlink:type="simple">akerke_toleugazykyzy@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5356-7874</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Болкенов</surname><given-names>Б. Т.</given-names></name><name name-style="western" xml:lang="en"><surname>Bolkenov</surname><given-names>B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Бакытжан Тургазыевич Болкенов – PhD, преподаватель профессор; программа «Туризм и гостеприимство», </p><p>010000, Aстана, Коргалжинское шоссе, 8</p></bio><bio xml:lang="en"><p>Bakytzhan Bolkenov – PhD, teaching professor, Tourism and Hospitality program,</p><p>010000, Astana, Korgalzhinskoe shosse, 8</p></bio><email xlink:type="simple">b_bolkenov@kazguu.kz</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5948-2636</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Искаков</surname><given-names>Р. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Iskakov</surname><given-names>R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Руслан Маратбекович Искаков – и.о. профессора кафедры «Технологические машины и оборудования»,</p><p> </p></bio><bio xml:lang="en"><p>Ruslan Iskakov – acting professor of the department «Technological machines and equipment»,</p><p>010000, Astana, Zhenis avenue, 62</p></bio><email xlink:type="simple">rus.iskakov79@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3504-3348</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Казангапова</surname><given-names>Н. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Kazangapova</surname><given-names>N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Нургуль Буркитбаевна Казангапова – PhD, кандидат географических наук, старший преподаватель кафедры лесных ресурсов и лесного хозяйства; </p><p>010000, Aстана, проспект Жеңіс, 62</p></bio><bio xml:lang="en"><p>Nurgul Kazangapova – PhD, candidate of geographical sciences, senior lecturer of the department «Forest Resources and Forestry»,</p><p>010000, Astana, Zhenis avenue, 62</p></bio><email xlink:type="simple">n.kazangapova@kazatu.edu.kz</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru">Казахский агротехнический исследовательский университет имени С.Сейфуллина<country>Казахстан</country></aff><aff xml:lang="en">S. Seifullin Kazakh Agrotechnical Research University<country>Kazakhstan</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru">Университет КАЗГЮУ имени М.С. Нарикбаева<country>Казахстан</country></aff><aff xml:lang="en">Maqsut Narikbayev University<country>Kazakhstan</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>27</day><month>09</month><year>2024</year></pub-date><volume>0</volume><issue>3(15)</issue><fpage>197</fpage><lpage>204</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Бекбаев К.С., Tөлеуғазықызы А., Болкенов Б.Т., Искаков Р.М., Казангапова Н.Б., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Бекбаев К.С., Tөлеуғазықызы А., Болкенов Б.Т., Искаков Р.М., Казангапова Н.Б.</copyright-holder><copyright-holder xml:lang="en">Bekbayev K., Toleugazykyzy A., Bolkenov B., Iskakov R., Kazangapova N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://tech.vestnik.shakarim.kz/jour/article/view/1275">https://tech.vestnik.shakarim.kz/jour/article/view/1275</self-uri><abstract><p>Валоризация лигноцеллюлозной биомассой (ЛБ) имеет важное значение для снижения их воздействия на окружающую среду и снижения опасности для здоровья человека. Традиционные подходы к обработке вторичных сырьевых материалов в основном сосредоточены на утилизации, рассматривая лигноцеллюлозную биомассу как отход, а не как ценный ресурс для создания продуктов с добавленной стоимостью. В качестве альтернативы следует разработать процессы добавления стоимости отходам, производящим продукты с добавленной стоимостью, имеющие экономические и экологические преимущества. В этой связи, исследования были сосредоточены на рабочих параметрах, предварительной обработке и микробной ферментации с целью увеличения выхода водорода во время темной ферментации. Установлены верхние (4%) и нижние (20%) концентрации субстратов на основе барды, при котором происходит синтез биоводорода с применением E. coli дикого типа. Установлены условия для рационального формирования редуцирующих сахаров за счет вариации концентраций сырья и кислоты в субстратах на основе послеспиртовой зерновой барды. В ходе исследования воздействия кислотно-гидротермальной обработки барды на общий выход биоводорода было установлено, что оптимальная концентрация серной кислоты составляет 1,5%, при этом используется 10% доля сырья. При таких условиях был достигнут максимальный выход молекулярного водорода, равный 116 ±1,0 мл/л с применением E. coli дикого типа.</p></abstract><trans-abstract xml:lang="en"><p>Valorization of lignocellulosic biomass (LB) is important to reduce their environmental impact and reduce the risk to human health. Conventional methods for handling secondary raw materials primarily focus on waste disposal, treating lignocellulosic biomass as waste rather than as a source of organic substances for producing value-added products. As an alternative, processes should be developed to add value to waste, producing value-added products with economic and environmental benefits. In this regard, studies have focused on operating parameters, pretreatment, and microbial fermentation to enhance hydrogen yield during dark fermentation. Upper (4%) and lower (20%) concentrations of distillery grain based substrates for biohydrogen synthesis using wild-type E. coli have been established. Conditions for rational formation of reducing sugars by varying feedstock and acid concentrations in distillery grain based substrates have been established. During the study of the effect of acid-hydrothermal treatment of stillage on the total yield of biohydrogen, it was found that the optimal concentration of sulfuric acid is 1,5%, while 10% of the raw material is used. Under these conditions, the maximum yield of molecular hydrogen was achieved, equal to 116 ±1,0 ml/l using wild-type E. coli.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>валоризация</kwd><kwd>послеспиртовая барда</kwd><kwd>предварительная обработка</kwd><kwd>темновая ферментация</kwd><kwd>сахара</kwd><kwd>молекулярный водород</kwd></kwd-group><kwd-group xml:lang="en"><kwd>valorization</kwd><kwd>distiller’s grains</kwd><kwd>pre-treatment</kwd><kwd>dark fermentation</kwd><kwd>sugars</kwd><kwd>molecular hydrogen</kwd></kwd-group><funding-group xml:lang="en"><funding-statement>This research has been/was/is funded by the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan (Grant No. AP19677558).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Environmental and human health impact of disposable face masks during the COVID-19 pandemic: wood-feeding termites as a model for plastic biodegradation / R. 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