kaz44Вестник Университета Шакарима. Серия технические наукиBulletin of Shakarim University. Technical Sciences2788-79953006-0524«Шәкәрім университеті» КеАҚ10.53360/2788-7995-2024-3(15)-14kaz44-1114Research ArticleПИЩЕВАЯ ИНЖЕНЕРИЯ И БИОТЕХНОЛОГИЯ (ОРИГИНАЛЬНАЯ СТАТЬЯ)FOOD ENGINEERING AND BIOTECHNOLOGY (ORIGINAL ARTICLE)ВЫДЕЛЕНИЕ И ИДЕНТИФИКАЦИЯ ШТАММОВ БАКТЕРИЙ, РАЗЛАГАЮЩИХ ЛИГНИНISOLATION AND IDENTIFICATION OF LIGNIN-DEGRADING BACTERIAL STRAINSЦяньиТяньQianyiTian

Цяньи Тянь – магистр биологии и медицины, студент,

712100, Янлин, Шэньси, 22 Xinong Road

Qianyi Tian – master of Biology and Medicine, student,

712100, Yangling, Shaanxi, 22 Xinong Road

1070567104@qq.comЛейДенгLeiDeng

Лей Дэн – магистр биологии и медицины, студент,

712100, Янлин, Шэньси, 22 Xinong Road

 

Lei Deng – master of Biology and Medicine, student,

712100, Yangling, Shaanxi, 22 Xinong Road

dengl@nwafu.edu.cnМиИньMiYin

Ми Инь – бакалавр,

712100, Янлин, Шэньси, 22 Xinong Road

Mi Yin – bachelor,

712100, Yangling, Shaanxi, 22 Xinong Road

1485742629@qq.comЯхонгВэйYahongWei

Яхонг Вэй – PhD, Факультет биоинженерии,

712100, Янлин, Шэньси, 22 Xinong Road

Yahong Wei – PhD, Department of Bioengineering, 

712100, Yangling, Shaanxi, 22 Xinong Road

yahongwei@nwafu.edu.cnКолледж естественных наук, Северо-Западный университет сельского хозяйства и лесоводстваКитайCollege of Life Sciences, Northwest A&F UniversityChina20242709202403(15)97104Copyright © Цяньи Т., Лей Д., Ми И., Яхонг В., 20242024Цяньи Т., Лей Д., Ми И., Яхонг В.Qianyi T., Lei D., Mi Y., Yahong W.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://tech.vestnik.shakarim.kz/jour/article/view/1114

Отбор штаммов бактерий, способных расщеплять лигнин, крайне необходим для переработки отходов сельского и лесного хозяйства. В ходе этого эксперимента мы собрали образцы почвы в горах Циньлин в Китае и выделили 99 штаммов бактерий. Далее, 18 штаммов бактерий были отобраны с использованием щелочной лигниновой твердой среды и культивировались при 30°C в течение 48 часов, после чего они были молекулярно идентифицированы. После этого для культивирования во встряхиваемой колбе использовали жидкую культуральную среду с лигнином, а для измерения изменений поглощения лигнина в видимом свете при 280 нм до и после культивирования использовали считыватель микропланшетов. В общей сложности было отобрано 12 штаммов бактерий, обладающих способностью к разложению лигнина, соответственно. Для QL-D3, QL-D5, QL-D6, QL-D7, QL-D8, QL-D9, QL-D11, QL-D13, QL-D14, QL-D16, QL-D17, QL -D18. Их скорость разложения лигнина после культивирования в жидкой среде с содержанием лигнина 1,5 г/л в течение 7 дней составила 13,01%, 16,76%, 10,48%, 23,3%, 6,88%, 12,48%, 22,07%, 11,84%, 18,62%, 17,88%, 13,95%, 16,28% соответственно. С помощью сканирующей электронной микроскопии была изучена морфология двух штаммов бактерий QL-D7 и QL-D11 с наибольшей скоростью разложения лигнина.

Screening bacterial strains with lignin-degrading ability is very necessary for processing agricultural and forestry waste. In this experiment, we collected soil samples from the Qinling Mountains in China and isolated 99 bacterial strains. Further, 18 strains of bacteria were screened out using alkaline lignin solid medium and cultured at 30°C for 48 hours, and they were molecularly identified. After that, lignin liquid culture medium was used for shake flask culture, and a microplate reader was used to measure the absorbance changes of lignin at visible light at 280 nm before and after culture. A total of 12 strains of bacteria with lignin degradation ability were screened out, respectively. For QL-D3, QL-D5, QL-D6, QL -D7, QL-D8, QL-D9, QL-D11, QL-D13, QL-D14, QL-D16, QL-D17, QL -D18. Their lignin degradation rates after culturing in lignin 1,5 g/L liquid medium for 7 days were 13,01%, 16,76%, 10,48%, 23,3%, 6,88%, 12,48%, 22,07%, 11,84%, 18,62%, 17,88%, 13,95%, 16,28% respectively. Scanning electron microscopy was used to observe the morphology of the two bacterial strains QL-D7 and QL-D11 with the highest lignin degradation rate.

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The authors declare that there are no conflicts of interest present.